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To come the toolset for controlling plant gene expression, we developed a CRISPR interference-based utterly platform for the constructing of synthetic Boolean logic gates that is useful in a pair of plant species. These genetic circuits are programmable and reversible in nature, which may possibly enable spatiotemporal management of plant responses to dynamic cues.
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References
Gaber, R. et al. Designable DNA-binding domains enable constructing of logic circuits in mammalian cells. Nat. Chem. Biol. 10203–208 (2014). This paper describes the constructing of logic gates in human cell lines utilizing TALE-based utterly repressors.
Kiani, S. et al. CRISPR transcriptional repression devices and layered circuits in mammalian cells. Nat. Suggestions 11723–726 (2014). This see reports the constructing of CRISPRi-based utterly layered devices in human cell lines.
Nielsen, A. A. Okay. & Voigt, C. A. Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol. Syst. Biol. 10763 (2014). This paper describes the interfacing of CRISPRi-based utterly circuits with host endogenous pathways in Escherichia coli.
Lloyd, J. P. B. et al. Synthetic memory circuits for real cell reprogramming in plant life. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01383-2 (2022). This be taught article describes the constructing of memory genetic circuits in plant life utilizing recombinases.
Brophy, J. A. N. et al. Synthetic genetic circuits as a technique of reprogramming plant roots. Science 377747–751 (2022). This paper reports the utilize of bacterial DNA-binding domains for constructing of logic gates in plant life.
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Right here’s a summary of: Khan, M. A. et al. CRISPRi-based utterly circuits to management gene expression in plant life. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02236-w (2024).
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Advancing programmable gene expression in plant life utilizing CRISPRi-based utterly Boolean gates. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02273-5
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DOI: https://doi.org/10.1038/s41587-024-02273-5
