Binary vector copy number engineering improves -mediated transformation
Technology tamfitronics MainAgrobacterium-mediated transformation (AMT) is an indispensable tool in both plant and fungal biotechnology for transgene insertion into target cells1,2,3. By replacing the native oncogenic genes used by the bacterial pathogen Agrobacterium tumefaciens with user-defined DNA sequences, the innate DNA transfer capacity of this bacterium can be exploited for untargeted DNA insertions into diverse plant, fungal and mammalian cell lines4,5,6,7. Initial improvements to AMT involved relocating two DNA sequences known as the left and right border (LB and RB) from the native ~200-kb tumor-inducing (Ti) plasmid to a smaller helper plasmid known as a binary vector8. Any DNA contained between the LB and RB is mobilized as a transfer DNA (T-DNA) and inserted into...
