Binary vector copy number engineering improves -mediated transformation
05 Nov
Science & Technology

Binary vector copy number engineering improves -mediated transformation

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Technology tamfitronics MainAgrobacterium-mediated transformation (AMT) is an indispensable tool in both plant and fungal biotechnology for transgene insertion into target cells1,2,3. By replacing the native oncogenic genes used by the bacterial pathogen Agrobacterium tumefaciens with user-defined DNA sequences, the innate DNA transfer capacity of this bacterium can be exploited for untargeted DNA insertions into diverse plant, fungal and mammalian cell lines4,5,6,7. Initial improvements to AMT involved relocating two DNA sequences known as the left and right border (LB and RB) from the native ~200-kb tumor-inducing (Ti) plasmid to a smaller helper plasmid known as a binary vector8. Any DNA contained between the LB and RB is mobilized as a transfer DNA (T-DNA) and inserted into...
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AIntibody: an experimentally validated in silico antibody discovery design challenge
05 Nov
Science & Technology

AIntibody: an experimentally validated in silico antibody discovery design challenge

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Technology tamfitronics Science is frequently subject to the Gartner hype cycle1: emergent technologies spark intense initial enthusiasm with the recruitment of dedicated scientists. As limitations are recognized, disillusionment often sets in; some scientists turn away, disappointed in the inability of the new technology to deliver on initial promise, while others persevere and further develop the technology. Although the value (or not) of a new technology usually becomes clear with time, appropriate benchmarks can be invaluable in highlighting strengths and areas for improvement, substantially speeding up technology maturation. A particular challenge in computational engineering and artificial intelligence (AI)/machine learning (ML) is that benchmarks and best practices are uncommon, so it is particularly hard for non-experts to...
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What will it take to get miRNA therapies to market?
05 Nov
Science & Technology

What will it take to get miRNA therapies to market?

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Technology tamfitronics The Nobel Prize in medicine was awarded for the discovery of miRNA, but miRNA therapeutics have a long way to go before they outcompete other therapies. The Nobel Prize in Medicine was awarded in early October to Victor Ambros and Gary Ruvkun for discovering how microRNAs (miRNAs) have central roles in gene expression by binding to target mRNAs and inhibiting their translation. Since their discovery in 1993, miRNAs have been found to influence gene expression pathways in cell differentiation, proliferation, and survival. Years of research have been done to understand how genetic pathways are directly influenced by miRNA binding and how these pathways are interrupted when miRNA expression becomes abnormal, leading to disease. However,...
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