Tag Archives: protein
Global profiling of protein complex dynamics with an experimental library of protein interaction markers
Technology tamfitronics MainMost biological processes are executed by proteins, often as part of dynamic protein complexes that assemble, disassemble or rearrange in response to environmental changes or internal cues. The composition and structure of protein complexes affect protein function, and the state of a cell is dictated by an interconnected network of protein–protein interactions (PPIs)1. Alterations in PPI networks can cause disease2,3,4,5,6,7,8,9,10and disease-associated mutations are enriched at protein-binding interfaces (PBIs)11. Disrupting12 and stabilizing13 PPIs is gaining increasing pharmacological relevance14. Further, profiling protein complexes and their dynamics is key to understanding and predicting biological systems.Affinity-purification mass spectrometry (AP–MS) and chromatographic fractionation mass spectrometry (CF–MS) have revealed a wealth of PPIs. For instance, an AP–MS study reported...
