Tag Archives: activity
Removal of TREX1 activity enhances CRISPR–Cas9-mediated homologous recombination
Technology tamfitronics MainRepair of CRISPR–Cas9-induced double-strand breaks (DSBs) occurs by one of two main mechanisms: non-homologous end joining (NHEJ) or homology-directed repair (HDR)1,2,3. The HDR pathway is used to introduce sequences of up to 2–3 kilobases (kb) into the genome that are supplied on an exogenous DNA template. In most human cells, the efficiency of HDR is very low relative to NHEJ4,5. Cellular context plays an important role in HDR efficiency. HDR is cell cycle limited and most active in S/G2 (refs. 6,7,8). Differential expression, cellular background and/or mutational burden can also affect efficiency. CRISPR–Cas9-mediated targeting of the same locus with identical reagents in various cell types can result in variable efficiency, ranging from 30%...
Mfg activity recovers in June: Survey
Business new tamfitronics TNN / Updated: Jul 2, 2024, 02:31 ISTAAText SizeSmallMediumLarge NEW DELHI: Growth in manufacturing activity in the country recovered in June on the back of solid demandtriggering the fastest rate of job creation in the sector in 19 years, a survey showed on Monday.The HSBC India Manufacturing Purchasing Managers' Index increased from 57.5 in May to 58.3 in June, indicating a sharper improvement in business conditions.The 50-point mark separates expansion from contraction in the survey, which is compiled from responses to questionnaires sent to 400 manufacturing companies.Manufacturing output increased at a sharp pace that was faster than in May, as underlying demand remained favourable and new business continued to flow in, the survey results showed. It said the performance of the consumer goods industry was especially strong, although...
